Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis
We successfully developed a method for isolation of brain microvessels from a small amount of frozen human brain tissue, facilitating detailed study of BBB proteome in aging or pathological conditions. This technique provides valuable insights into BBB dysfunction in central nervous system disorders and holds potential for improving brain-targeted drug delivery strategies.
Authors
- Seiryo Ogata (ORCID: https://orcid.org/0000-0001-5422-7823)
- Takeshi Masuda (ORCID: https://orcid.org/0000-0003-0121-4783)
- Sumio Ohtsuki (ORCID: https://orcid.org/0000-0003-4634-7133)
- Shingo Ito (ORCID: https://orcid.org/0000-0002-8862-471X)
Institutions
- Tohoku University (JP)
- National Institute of Technology, Tsuruoka College (JP)
- Kumamoto University (JP)
Publication Details
- Journal
- Fluids and Barriers of the CNS
- Published
- 2024-12-19
- DOI
- https://doi.org/10.1186/s12987-024-00609-6
- Citations
- 2
- Primary Topic
- Barrier Structure and Function Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.49
Funders
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science