Thermoresponsive gel embedding extracellular vesicles from adipose stromal cells improves the healing of colonic anastomoses following irradiation in rats
Anastomotic leak occurrence is a severe complication after colorectal surgery. Considering the difficulty of treating these leaks and their impact on patient care, there is a strong need for an efficient prevention strategy. We evaluated a combination of extracellular vesicles (EVs) from rat adipose-derived stromal cells with a thermoresponsive gel, Pluronic® F127 (PF-127) to prevent anastomotic leaks. The pro-regenerative and immunomodulatory potencies of EVs are assessed in vitro. In vivo efficacy are assessed in rat with a colonic anastomosis model after irradiation. Endoscopic, anatomical and histological data show a consistent effect of EVs + gel on the healing of colonic anastomosis. These results are illustrated by a smaller anastomotic ulcer size, less fibrosis and less inflammatory infiltrations in the EVs + gel group. This multi-modal investigation is the first to point-out the translational potential of EVs combined with PF-127 for the healing of high-risk colorectal anastomosis.
Authors
- Gabriel Rahmi (ORCID: https://orcid.org/0000-0002-9452-1450)
- Anna Sebbagh (ORCID: https://orcid.org/0000-0002-9717-2444)
- Christelle Demarquay
- Claire Wilhelm (ORCID: https://orcid.org/0000-0001-7024-9627)
- Arthur Berger (ORCID: https://orcid.org/0000-0003-0387-4734)
- Guillaume Péré (ORCID: https://orcid.org/0000-0002-1212-0339)
- Olivier Clémеnt (ORCID: https://orcid.org/0000-0002-6890-671X)
- Irami Araújo-Filho (ORCID: https://orcid.org/0000-0003-2471-7447)
- N. Mathieu (ORCID: https://orcid.org/0000-0002-5753-7154)
- Florence Gazeau (ORCID: https://orcid.org/0000-0002-6482-3597)
- Hadrien Alric (ORCID: https://orcid.org/0000-0002-0343-4440)
- M. Karoui (ORCID: https://orcid.org/0000-0001-9255-4032)
- Antoine Mariani (ORCID: https://orcid.org/0000-0002-9626-0004)
- B. Marcel
- Amanda Silva (ORCID: https://orcid.org/0000-0002-7713-1813)
- André Cronemberger
Institutions
- Institut de Radioprotection et de Sûreté Nucléaire (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Université Paris Cité (FR)
- Université Paris Sciences et Lettres (FR)
- Centre Hospitalier Universitaire de Bordeaux (FR)
- Centre Hospitalier Universitaire de Toulouse (FR)
- Universidade Federal do Rio Grande do Norte (BR)
- Sorbonne Université (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Hôpital Européen Georges-Pompidou (FR)
- Hôpital Européen (FR)
- Hôpital Rangueil (FR)
- Laboratoire Matière et Systèmes Complexes (FR)
- Paris Cardiovascular Research Center (FR)
- Physique des Cellules et Cancers (FR)
- Institut Curie (FR)
Publication Details
- Journal
- Communications Biology
- Published
- 2024-12-19
- DOI
- https://doi.org/10.1038/s42003-024-07364-2
- Citations
- 1
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- Agence Nationale de la Recherche