Structural and functional alterations of neurons derived from sporadic Alzheimer’s disease hiPSCs are associated with downregulation of the LIMK1-cofilin axis
Our findings demonstrate that although sAD- and HS-hiPSCs are similar in their stemness and genomic stability, sAD-hNs exhibit distinct functional and structural anomalies mirroring AD pathology. These anomalies include synaptic dysfunction, altered cytoskeletal organization, and accumulation of AD-related proteins. Our study underscores the usefulness of hiPSCs in modeling AD and provides insights into the disease's molecular underpinnings, thus highlighting potential therapeutic targets.
Authors
- Agnese Novelli (ORCID: https://orcid.org/0009-0008-4249-0841)
- Guido Maria Giuffrè (ORCID: https://orcid.org/0000-0002-7271-1229)
- Jessica Rosati (ORCID: https://orcid.org/0000-0002-4483-386X)
- Fabiola Paciello (ORCID: https://orcid.org/0000-0002-8473-8074)
- Claudio Grassi (ORCID: https://orcid.org/0000-0001-7253-1685)
- Giuseppe Aceto (ORCID: https://orcid.org/0000-0002-7530-6835)
- Francesco Pastore (ORCID: https://orcid.org/0000-0003-0064-1505)
- Claudia Colussi (ORCID: https://orcid.org/0000-0002-7727-8911)
- Cristian Ripoli (ORCID: https://orcid.org/0000-0002-5315-0163)
- Camillo Marra (ORCID: https://orcid.org/0000-0003-3994-4044)
- Raimondo Sollazzo (ORCID: https://orcid.org/0000-0002-6594-231X)
- Domenica Donatella Li Puma (ORCID: https://orcid.org/0000-0001-6729-6967)
- Maria Gabriella Vita (ORCID: https://orcid.org/0000-0002-6547-4990)
- Francesco Danilo Tiziano (ORCID: https://orcid.org/0000-0002-5545-6158)
- Alessia Casamassa (ORCID: https://orcid.org/0000-0001-6329-7153)
- Sabrina Maietta
Institutions
- Università Cattolica del Sacro Cuore (IT)
- Casa Sollievo della Sofferenza (IT)
- Agostino Gemelli University Polyclinic (IT)
- Saint Camillus International University of Health and Medical Sciences (IT)
- Istituti di Ricovero e Cura a Carattere Scientifico (IT)
- Istituto di Analisi dei Sistemi ed Informatica Antonio Ruberti (IT)
Publication Details
- Journal
- Alzheimer s Research & Therapy
- Published
- 2024-12-19
- DOI
- https://doi.org/10.1186/s13195-024-01632-3
- Citations
- 7
- Primary Topic
- Alzheimer's disease research and treatments
- Type
- article
- Field-Weighted Citation Impact
- 2.29
Funders
- Alzheimer's Association
- Ministero della Salute