Protocol for X-ray irradiation of C57BL/6J recipient mice followed by the transplantation of mTomato-expressing bone marrow cells
Bone marrow chimera is a useful tool to determine the pathophysiological contributions of hematopoietic versus stromal compartments. Here, we present a protocol for lethal irradiation of wild-type C57BL/6J recipient mice followed by the transplantation of bone marrow from mTomato-expressing donors. We then detail procedures for animal distress scoring and assessment of reconstitution efficiency. This protocol will minimize distress and ensure efficient hematopoietic reconstitution of donor cells in recipients.
Authors
- Ishita Choudhary (ORCID: https://orcid.org/0000-0001-8242-3928)
- Kshitiz Paudel (ORCID: https://orcid.org/0000-0001-7125-8381)
- Markus Metzler (ORCID: https://orcid.org/0000-0002-4523-1676)
- Amit Sharma (ORCID: https://orcid.org/0000-0001-5037-1043)
- Amol Suryawanshi (ORCID: https://orcid.org/0000-0001-5031-5818)
- Michael W. Nolan (ORCID: https://orcid.org/0000-0002-4432-9700)
- Yogesh Saini (ORCID: https://orcid.org/0000-0002-8324-2122)
- Sonika Patial (ORCID: https://orcid.org/0000-0002-7373-0835)
- Thao Vo (ORCID: https://orcid.org/0009-0005-5439-8354)
- Richa Lamichhane (ORCID: https://orcid.org/0009-0003-0722-658X)
- Rahul Kumar (ORCID: https://orcid.org/0000-0002-0934-0859)
- Yun Mao
- Rekha Kc
Institutions
- North Carolina State University (US)
- National Institute of Environmental Health Sciences (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-18
- DOI
- https://doi.org/10.1016/j.xpro.2024.103504
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health
- National Institute of Environmental Health Sciences