Volumetric imaging of trabecular meshwork dynamic motion using 600 kHz swept source optical coherence tomography

The motion of the trabecular meshwork (TM) facilitates the aqueous drainage from the anterior chamber to the venous system, thereby maintaining normal intraocular pressure. As such, characterizing the TM motion is valuable for assessing the functionality of the aqueous outflow system, as demonstrated by previous phase-sensitive optical coherence tomography (OCT) studies. Current methods typically acquire motion from a single cross-sectional plane along the circumference of the anterior chamber. While effective, the lateral scan pattern only intersects one spatial location on the TM at a time, significantly limiting examination throughput. In this study, we introduce the first volumetric imaging approach for assessing TM motion. Rather than monitoring a single cross-sectional plane, our method employs repeated volumetric scans, allowing for simultaneous observation of a continuous TM band spanning two millimeters. We also show that the field of view could be further expanded by stitching multiple scans. To ensure robust data processing, we developed a customized volume registration algorithm to correct motion artifacts and an automated segmentation algorithm to identify the TM boundary based on the correlation of OCT phase dynamics with heartbeats. Imaging results from a healthy subject confirmed the feasibility of our approach, revealing considerable variation in TM motions at different spatial locations through the stitching process. This proposed methodology offers unprecedented capabilities and examination throughput in the biomechanical imaging of the TM, providing significant scientific insights and diagnostic value for identifying abnormalities in aqueous outflow.