Antifibrotic Drug Delivery for Glaucoma Treatment – Determination of Josamycin in vivo

Abstract Antifibrotic treatment during glaucoma surgery leads to an improved outcome for patients. Drug delivery systems for placement under the subconjuctiva after surgery made of cyclodextrins and crosslinked with Epichlorohydin were loaded with Josamycin and tested in vivo. After subconjunctival application in three New Zealand White rabbits, blood samples were taken by the means of Mitra devices at defined time points. Determination of blood levels of Josamycin was performed using liquid chromatography mass spectrometry. Detection of Josamycin was achieved during 48 h suggesting a sustained release over the course of this study.