Transcriptome and Gene Expression Analysis Revealed CeNA1: A Potential New Marker for Somatic Embryogenesis in Common Centaury (Centaurium erythraea Rafn.)

<i>Centaurium erythraea</i> Rafn. is a medicinal plant used as a model for studying plant developmental processes due to its developmental plasticity and ease of manipulation in vitro. Identifying the genes involved in its organogenesis and somatic embryogenesis (SE) is the first step toward unraveling the molecular mechanisms underlying its morphogenic plasticity. Although SE is the most common method of centaury regeneration, the genes involved in this have not yet been identified. The aim of this study was to identify the differentially expressed genes (DEGs) during key stages of SE and organogenesis using transcriptome data, with a focus on novel SE-related genes. The transcriptomic analysis revealed a total of 4040 DEGs during SE and 12,708 during organogenesis. Gene Ontology (GO) annotation showed that the highest number of SE-related genes was involved in defense responses. The expression of fifteen selected SE-related candidate genes was assessed by RT-qPCR across nine centaury developmental stages, including embryogenic tissues. Notably, a newly reported transcript, named <i>CeNA1</i>, was specifically activated during embryogenic callus (<b>ec</b>) induction, making it a potential novel marker for early SE. These findings provide, for the first time, insight into SE-related transcriptional patterns, representing a step closer to uncovering the molecular basis of centaury's developmental plasticity.