Endurance Effort Affected Expression of Actinin 3 and Klotho Different Isoforms Basing on the Arabian Horses Model

Blood samples were taken from a group of <i>n</i> = 10 Arabian horses taking part in a long-distance 120 km endurance ride. After RNA isolation and reverse transcription, real-time PCR was performed. The expression levels (Relative Quantity, RQ) were calculated using the delta-delta CT method. The results showed surprisingly large differences between different isoforms expression levels which brought us to the conclusion that both <i>KL</i> and <i>ACTN3</i> genes are suitable genetic markers to measure endurance performance. Moreover, the correlation network analyses showed that the <i>MIOX</i> (myo-inositol oxygenase), <i>SH3RH2</i> (SH3 domain-containing ring finger 2) and <i>TNNI2</i> (Troponin I2, fast skeletal type) genes are significantly involved in the endurance effort metabolism.