A Statistical Approach for Cellulase Optimization in Strain Aspergillus Tubingensis Isolated from Cowbarn Area.

Cellulases would prove to find vast array of applications in many industries due to which they will attain sturdy attention of many researchers throughout the world. The purpose of this study was to investigate and employ the statistical methods for cellulase production in Aspergillus tubingensis fungal strain isolated from soil of cowbarn area. The medium components enhancing the cellulase production were optimized by one factor at a time methodology (OFAT), Plackett Burman methodology (PB) and Response Surface methodology (RSM) using Box Behnken Design (BBD). Eight factors were selected in Plackett Burman (PB) methodology after OFAT for optimization of cellulase production. Among 8 factors, Carboxy Methyl Cellulose (CMC), Yeast Extract and Ammonium Chloride (NH4Cl) into the culture medium were selected due to significant positive effect on cellulase yield. Box Behnken Design(BBD), a response surface methodology, was used for further optimization of these selected factors for better cellulase activity. The media formulations were optimized having the factors such as Carboxy Methyl Cellulose (CMC) 20g/L, Yeast extract 0.5g/L and Ammonium Chloride (NH4Cl) 0.3g/L which led to the fine tuning of the cellulase production and enhancing cellulase activity from 2.22 U/ml to 41.925 U/ml.