Rapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay
Our results have brought proof of principle on the usefulness of the one-step fast multiplex RT- PCR assay coupled to PCRD as a new assay for specific, sensitive, and rapid detection of SARS-CoV-2 without requiring costly laboratory equipment, and thus, at reduced costs in a format prone to be deployed when resources are limited. This assay offers a viable alternative for COVID-19 diagnosis or screening at points of need.
Authors
- Hejer Souguir (ORCID: https://orcid.org/0000-0002-4455-5900)
- Kaouther Ayouni (ORCID: https://orcid.org/0000-0002-0872-5706)
- Insaf Bel Hadj Ali (ORCID: https://orcid.org/0000-0002-5289-5892)
- M. Ardhaoui
- S Boubaker (ORCID: https://orcid.org/0000-0001-9705-4387)
- Henda Triki
- Yusr Saadi Ben Aoun (ORCID: https://orcid.org/0000-0001-5850-4218)
- Ikram Guizani (ORCID: https://orcid.org/0000-0003-2763-8963)
- Mohamed Vall Taleb Mohamed
- Mouna Melliti
Institutions
- Tunis University (TN)
- Institut Pasteur de Tunis (TN)
- Tunis El Manar University (TN)
Publication Details
- Journal
- BMC Infectious Diseases
- Published
- 2024-12-18
- DOI
- https://doi.org/10.1186/s12879-024-10296-1
- Citations
- 4
- Primary Topic
- SARS-CoV-2 detection and testing
- Type
- article
- Field-Weighted Citation Impact
- 1.56
Funders
- Ministère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
- Institut Pasteur de Tunis