Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers
By engineering PP<sub>i</sub>-free glycolysis in C. thermocellum, we achieved an increase in ethanol production. These results demonstrate that optimizing the thermodynamic landscape through metabolic engineering can enhance product titers. While further increases in ethanol titers are necessary for commercial application, this work represents a significant step toward engineering glycolysis in C. thermocellum to increase ethanol titers.
Authors
- David Stevenson (ORCID: https://orcid.org/0000-0001-9117-138X)
- Daniel G. Olson (ORCID: https://orcid.org/0000-0001-5393-6302)
- Bishal Dev Sharma (ORCID: https://orcid.org/0000-0002-8326-4444)
- Daniel Amador‐Noguez (ORCID: https://orcid.org/0000-0002-3568-3070)
- Lee R. Lynd (ORCID: https://orcid.org/0000-0002-5642-668X)
- Shuen Hon (ORCID: https://orcid.org/0000-0003-2146-0105)
- Adam M. Guss (ORCID: https://orcid.org/0000-0001-5823-5329)
- Eashant Thusoo
Institutions
- Dartmouth College (US)
- Oak Ridge National Laboratory (US)
- University of Wisconsin–Madison (US)
- Great Lakes Bioenergy Research Center (US)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2024-12-18
- DOI
- https://doi.org/10.1186/s13068-024-02591-5
- Citations
- 10
- Primary Topic
- Biofuel production and bioconversion
- Type
- article
- Field-Weighted Citation Impact
- 1.84
Funders
- U.S. Department of Energy
- Joint Genome Institute
- Office of Science
- Biological and Environmental Research
- Center for Bioenergy Innovation