Generation of INS-jGCaMP7f knock-in Ca2+ reporter human embryonic stem cell line, GZLe001-C, using CRISPR/Cas9-based gene targeting
As a member of the single-fluorophore genetically encoded calcium indicators (GECIs), jGCaMP7f is widely applied to investigate intracellular Ca<sup>2+</sup> concentrations. Here, we established an INS-jGCaMP7f knock-in H1 human embryonic stem cell (hESC) line by integrating jGCaMP7f gene into insulin locus via CRISPR/Cas9 system. The reporter cell line not only effectively labelled the insulin-producing cells induced from hESC, but also reflected the cytosolic change of Ca<sup>2+</sup> level in response to different stimuli. This reporter cell line is a valuable research tool for studying functional maturation of hESC-derived insulin-producing cells, conducting drug screenings, and exploring the mechanisms of diabetes.
Authors
- Haopeng Lin
- Tao Xu (ORCID: https://orcid.org/0000-0002-6962-3404)
- Wei Peng (ORCID: https://orcid.org/0000-0002-9572-951X)
- Qifei Dong (ORCID: https://orcid.org/0000-0002-5751-3208)
- Jiaxiang Yin (ORCID: https://orcid.org/0000-0001-5249-7483)
- Zirong Bi
- Feng Zhang (ORCID: https://orcid.org/0000-0003-0326-5365)
- Xin Liu (ORCID: https://orcid.org/0000-0003-0813-5164)
- Jiawei Yan
- Huisheng Liu (ORCID: https://orcid.org/0000-0001-5371-7943)
- Deqi Chen (ORCID: https://orcid.org/0000-0001-9854-9736)
- Yanying Guo (ORCID: https://orcid.org/0000-0001-7228-5656)
- Lihua Chen (ORCID: https://orcid.org/0000-0001-9000-5769)
Institutions
- Guangzhou University (CN)
- Guangzhou Experimental Station (CN)
- People's Hospital of Xinjiang Uygur Autonomous Region (CN)
- University of South China (CN)
- Guangzhou Medical University (CN)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2024-12-18
- DOI
- https://doi.org/10.1016/j.scr.2024.103633
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 0.00