Multi-faceted effects and mechanisms of granular activated carbon to enhance anaerobic ammonium oxidation (anammox) for nitrogen removal from wastewater

Nitrogen removal via anammox is efficient but challenged by their slow growth. Adding granular activated carbon (GAC) increased the total nitrogen removal rate to 66.99 g-N/m³/day, compared to 50.00 g-N/m³/day in non-GAC reactor. Both reactors dominated by Candidatus Brocadia (non-GAC: 36.25 %, GAC: 35.5 %) but GAC improved specific anammox activity. Functional metabolic profiling from metagenomic analysis unveiled that GAC enhanced pathways associated with electron shuttle production, potentially promoting intra/extracellular electron transfer. In nitrogen metabolism, GAC is indicated to facilitate anammox N₂H₄ synthesis process, and inhibit nitrification and full denitrification processes, functioned by Nitrosomonas and Castellaniella which are more abundant in the non-GAC reactor. GAC also enhanced dissimilatory nitrate reduction to ammonium and partial denitrification processes, providing anammox with NH₄⁺/NO, which was conducted by Anaerolineae members (29.7 % in GAC-reactor and 7.8 % in non-GAC reactor sludge). This research illuminated the intricate microbial nitrogen cycling networks affected by GAC in anammox systems.