Protocol for efficient generation of human artery and vein endothelial cells from pluripotent stem cells
Blood vessels permeate all organs and execute myriad roles in health and disease. Here, we present a protocol to efficiently generate human artery and vein endothelial cells (ECs) from pluripotent stem cells within 3-4 days of differentiation. We delineate how to seed human pluripotent stem cells and sequentially differentiate them into primitive streak, lateral mesoderm, and either artery or vein ECs. We differentiate stem cells in defined, serum-free culture media in monolayers, without feeder cells or genetic manipulations. For complete details on the use and execution of this protocol, please refer to Ang et al. <sup>1</sup>.
Authors
- Sherry Li Zheng (ORCID: https://orcid.org/0000-0002-1065-5073)
- Sawan Kumar Jha (ORCID: https://orcid.org/0000-0003-1898-4928)
- Kyle M. Loh (ORCID: https://orcid.org/0000-0002-8042-0149)
- Christine Cheung (ORCID: https://orcid.org/0000-0001-7127-9107)
- Alana Nguyen
- Kevin J. Liu (ORCID: https://orcid.org/0000-0003-3836-3213)
- Lay Teng Ang (ORCID: https://orcid.org/0000-0002-6783-4662)
- Edda Spiekerkoetter (ORCID: https://orcid.org/0000-0002-3868-5186)
- Vanessa Kristina Wazny (ORCID: https://orcid.org/0000-0002-5507-6729)
- Kristy Red‐Horse (ORCID: https://orcid.org/0000-0003-1541-601X)
- Qingqing Yin (ORCID: https://orcid.org/0000-0002-1055-8994)
- Angela Chen (ORCID: https://orcid.org/0000-0002-2697-0385)
- Yue Qi (ORCID: https://orcid.org/0000-0002-7699-5572)
- Faith-Masong Njunkeng
- Zhainib A Amir-Ugokwe (ORCID: https://orcid.org/0000-0003-1429-1729)
Institutions
- Howard Hughes Medical Institute (US)
- Nanyang Technological University (SG)
- California Institute for Regenerative Medicine (US)
- Pulmonary and Allergy Associates (US)
- Stanford Medicine (US)
- Institute of Molecular and Cell Biology (SG)
- Stanford University (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-18
- DOI
- https://doi.org/10.1016/j.xpro.2024.103494
- Citations
- 6
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 1.40
Funders
- National Science Foundation
- Howard Hughes Medical Institute
- California Institute for Regenerative Medicine
- Stanford Diabetes Research Center
- Additional Ventures
- National Institutes of Health
- Advanced Research Projects Agency
- Ludwig Center at Harvard
- Lee Kong Chian School of Medicine, Nanyang Technological University
- Stanford Maternal and Child Health Research Institute