Shaken, not stirred: magnetic bead DNA extraction as a rapid and effective method for the scaling up of bovine tuberculosis diagnosis
The results of this study indicate that the MagMAX CORE Nucleic Acid Purification kit coupled with a KingFisher Flex instrument is a valuable alternative for the extraction of MTBC DNA from bovine tissues. However, the increased variability and Ct values suggest that a larger amount of starting material is recommended for this methodology, warranting further studies.
Authors
- Lucía de Juan
- Beatriz Romero (ORCID: https://orcid.org/0000-0001-5263-1806)
- Alberto Gómez-Buendía (ORCID: https://orcid.org/0000-0002-8968-0836)
- Víctor Lorente-Leal (ORCID: https://orcid.org/0000-0003-4096-0885)
- Javier Bezos (ORCID: https://orcid.org/0000-0003-1913-0545)
- A Gutiérrez-Tobaruela
Institutions
- Universidad Complutense de Madrid (ES)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2024-12-19
- DOI
- https://doi.org/10.1186/s12917-024-04427-8
- Citations
- 3
- Primary Topic
- Mycobacterium research and diagnosis
- Type
- article
- Field-Weighted Citation Impact
- 1.16
Funders
- European Commission
- Ministerio de Agricultura, Pesca y Alimentación