Effect of oil and diesel fuel pollution on enzymatic activity of meadow chernozem-like soil

In the laboratory experiment, the effect of oil pollution on the short-term dynamics of the activity of urease, phosphatase, catalase, peroxidase and polyphenol oxidase of the chernozem soil of the south of the Far East was studied. It was found that when soil was contaminated with oil and petroleum products, urease activity decreased by 20–44% at the end of incubation, peroxidase activity increased by 39–49% in the middle and end of incubation, polyphenol oxidase activity increased 1.6–2.0 times in the middle of incubation. The activity of phosphatase and catalase was stable at different levels of contamination throughout the experiment. Examining the effect of the incubation period, it was found that the maximum activity of urease was on the 10th day, phosphatase – on the 20th, peroxidase – on the 20th and 30th, polyphenol oxidase – on the 30th day of the experiment. Catalase activity was stable throughout all incubation periods. It was shown that in the case of contamination of chernozem-like soil with oil and diesel fuel in doses up to 5000 mg/kg, inhibition of enzymatic activity did not occur in the early stages.