Bengtsoniella intestinalis gen. nov., sp. nov., a member of the family Oscillospiraceae, isolated from the hindgut of the marine herbivorous fish Kyphosus sydneyanus

A Gram-stain-negative, non-spore-forming, rod-shaped, obligately anaerobic bacterium, designated strain BP47G, was isolated from the hindgut of a silver drummer (<i>Kyphosus sydneyanus</i>) fish collected from the Hauraki Gulf, New Zealand. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that it belonged to the family <i>Oscillospiraceae</i> in the phylum <i>Bacillota</i>. The gene sequence of BP47G was most similar to <i>Oscillibacter valericigenes</i> with 95.23% sequence identity. Isolate BP47G grew on agar medium containing mannitol and fish gut fluid as the sole carbon sources. Clear colonies of ~1 mm diameter grew within a week at 20-28 °C (optimum 28 °C) and pH 7.1-8.5 (optimum 8.5). BP47G tolerated the addition to the medium of up to 1% NaCl. Formate and butyrate were the major fermentation products. The major cellular fatty acids were C_12:0, C_13:0, iso-C_14:0, C_16:0 and C_16:1 <i>cis</i> 7. Genomic analyses comparing BP47G with its closest relatives indicated low genomic relatedness based on the average nucleotide identity, average amino acid identity, percentage of conserved protein and <i>in silico</i> DNA-DNA hybridization. Supported by the phenotypic and taxonomic characteristics observed in this study, a novel genus and species <i>Bengtsoniella intestinalis</i> gen. nov., sp. nov. is proposed for isolate BP47G (=ICMP 24688=JCM 35770).