In-vivo Raman microspectroscopy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots
We present a methodology based on Raman microspectroscopy for in-vivo label-free mapping of nitrate within small root tissue volumes in Arabidopsis. Measurements are done in-situ without additional sample preparation. Our measurements revealed nitrate concentration changes from lower to higher concentration from tip to mature root tissue. Accumulation of nitrate in the maturation zone tissue shows a saturation behavior. The presented Raman-based approach allows for in-situ non-destructive measurements of Raman-active compounds.
Authors
- Aart Verhoef (ORCID: https://orcid.org/0000-0003-2691-214X)
- Ze Tian Fang (ORCID: https://orcid.org/0009-0003-5253-1823)
- Yukihiro Nagashima (ORCID: https://orcid.org/0000-0001-6074-2215)
- Brian Henrich
- Dipankar Sen (ORCID: https://orcid.org/0009-0009-4099-2175)
- Sokolov Av (ORCID: https://orcid.org/0000-0001-9033-0537)
- Sakiko Okumoto (ORCID: https://orcid.org/0000-0003-1451-5446)
- Ana María González (ORCID: https://orcid.org/0000-0002-9311-0967)
Institutions
- Texas A&M University System (US)
- Texas A&M University (US)
Publication Details
- Journal
- Plant Methods
- Published
- 2024-12-18
- DOI
- https://doi.org/10.1186/s13007-024-01302-3
- Citations
- 4
- Primary Topic
- Spectroscopy Techniques in Biomedical and Chemical Research
- Type
- article
- Field-Weighted Citation Impact
- 2.65
Funders
- U.S. Department of Energy
- Welch Foundation
- Texas A and M University