Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches
Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
Authors
- Jennifer A. Faralli
- Hannah Youngblood (ORCID: https://orcid.org/0000-0002-8326-7282)
- Colleen M. McDowell (ORCID: https://orcid.org/0000-0001-9462-3556)
- Colin E. Willoughby (ORCID: https://orcid.org/0000-0002-1246-4166)
- Donna M. Peters (ORCID: https://orcid.org/0000-0002-1303-7027)
- Markus H. Kuehn (ORCID: https://orcid.org/0000-0003-3940-198X)
- Darryl R. Overby (ORCID: https://orcid.org/0000-0001-9894-7515)
- Philip Mzyk (ORCID: https://orcid.org/0000-0003-2632-644X)
- Gavin W. Roddy (ORCID: https://orcid.org/0000-0002-8905-8588)
- David Križaj (ORCID: https://orcid.org/0000-0003-4468-3029)
- Diane E. Bovenkamp (ORCID: https://orcid.org/0000-0002-5814-5785)
- Vijay Krishna Raghunathan (ORCID: https://orcid.org/0000-0003-1702-591X)
- Myoung Sup Shim (ORCID: https://orcid.org/0000-0001-6535-1416)
- Alex S. Huang (ORCID: https://orcid.org/0000-0001-9024-9608)
- Preeti Subramanian
- Kate E. Keller (ORCID: https://orcid.org/0000-0002-1616-972X)
- Najam A. Sharif (ORCID: https://orcid.org/0000-0002-4432-730X)
- Samuel Herberg (ORCID: https://orcid.org/0000-0001-7196-2010)
- Gaurang Patel (ORCID: https://orcid.org/0000-0003-2497-665X)
- Fiona McDonnell (ORCID: https://orcid.org/0000-0001-7563-7258)
- Padmanabhan P. Pattabiraman (ORCID: https://orcid.org/0000-0003-1902-0493)
- Ruth A. Kelly (ORCID: https://orcid.org/0000-0001-8041-4411)
- Cynthia Steel
- Krishnakumar Kizhatil (ORCID: https://orcid.org/0000-0003-3755-6101)
- Michael P. Fautsch (ORCID: https://orcid.org/0000-0002-9115-2485)
- C. Ross Ethier (ORCID: https://orcid.org/0000-0001-6110-3052)
- Weiming Mao (ORCID: https://orcid.org/0000-0003-0597-1490)
- Yang Sun (ORCID: https://orcid.org/0000-0001-8735-4765)
- Murray Johnstone (ORCID: https://orcid.org/0000-0003-2136-2433)
- Michael Peng (ORCID: https://orcid.org/0000-0001-7121-7328)
- Cydney A. Wong (ORCID: https://orcid.org/0000-0001-9381-5847)
- Gulab Zode (ORCID: https://orcid.org/0000-0002-7823-2595)
- W. Daniel Stamer (ORCID: https://orcid.org/0000-0002-2504-8997)
- Mohammed Dibas
- Mini Aga (ORCID: https://orcid.org/0009-0001-2449-7141)
- Elizabeth White
- Haiyan Gong (ORCID: https://orcid.org/0000-0002-7204-5019)
- Rudolf Fuchshofer
- Kamisha R. Hill (ORCID: https://orcid.org/0000-0003-1575-3382)
- K. Saidas Nair (ORCID: https://orcid.org/0000-0001-5309-9727)
- Ester Reina‐Torres (ORCID: https://orcid.org/0000-0002-4572-2539)
- Joseph M. Sherwood (ORCID: https://orcid.org/0000-0002-4402-9767)
- Sanjoy K. Bhattacharya (ORCID: https://orcid.org/0000-0003-3759-647X)
- Michael H. Elliott (ORCID: https://orcid.org/0000-0002-2289-8718)
- Uttio Roy Chowdhury
- Alireza Karimi (ORCID: https://orcid.org/0000-0001-9881-611X)
- Gillian J. McLellan (ORCID: https://orcid.org/0000-0002-6505-7476)
- Raquel L. Lieberman (ORCID: https://orcid.org/0000-0001-9345-3735)
- Chenna Kesavulu Sugali (ORCID: https://orcid.org/0000-0002-6523-7688)
- Hao F. Zhang (ORCID: https://orcid.org/0000-0001-5089-1196)
- Paloma B. Liton (ORCID: https://orcid.org/0000-0002-1440-3762)
- Benjamin R. Thomson (ORCID: https://orcid.org/0000-0001-6565-5866)
- Terete Borrás
- Clemens Strohmaier (ORCID: https://orcid.org/0000-0001-8249-1138)
- Abbot F. Clark (ORCID: https://orcid.org/0000-0003-3594-6560)
- E. Griffen Samples
- Carol B. Toris (ORCID: https://orcid.org/0000-0003-3794-6142)
- Yiqin Du (ORCID: https://orcid.org/0000-0002-4330-0957)
- Ganesh Prasanna (ORCID: https://orcid.org/0000-0001-6420-270X)
- Yutao Liu (ORCID: https://orcid.org/0000-0003-2870-4504)
- Ponugoti Vasantha Rao (ORCID: https://orcid.org/0000-0001-5470-9785)
- Mary J. Kelley
- Hoi Lam Li
- Paul L. Kaufman
- Shan C. Lin (ORCID: https://orcid.org/0000-0001-5637-1853)
- Thomas F. Freddo
- Ted S. Acott
- John R. Samples
- Casey C. Kopcyznski
Institutions
- Boston University (US)
- Northwestern University (US)
- University of North Carolina at Chapel Hill (US)
- Johannes Kepler University of Linz (AT)
- University of North Texas (US)
- University of Iowa (US)
- Georgia Institute of Technology (US)
- Mayo Clinic (US)
- University of Wisconsin–Madison (US)
- University of Ulster (GB)
- University of Miami (US)
- University of North Texas Health Science Center (US)
- Oregon Health & Science University (US)
- Massachusetts College of Pharmacy and Health Sciences (US)
- Washington State University Spokane (US)
- Duke University (US)
- University of California, San Francisco (US)
- Regeneron (United States) (US)
- University of Washington (US)
- SUNY Upstate Medical University (US)
- University of California, Irvine (US)
- University of Utah (US)
- Augusta University (US)
- University of South Florida (US)
- The Wallace H. Coulter Department of Biomedical Engineering (US)
- The Ohio State University Wexner Medical Center (US)
- AbbVie (United States) (US)
- University of California San Diego (US)
- Aerie Pharmaceuticals (United States) (US)
- Santen (United States) (US)
- Novartis (United States) (US)
- Iowa City VA Health Care System (US)
- Eye Institute of Utah (US)
- Glaucoma Research Foundation (US)
- Indiana University School of Medicine
- Imperial College London (GB)
- University of Oklahoma Health Sciences Center (US)
- The Ohio State University (US)
- Indiana University – Purdue University Indianapolis (US)
- University of Regensburg (DE)
- Smith-Kettlewell Eye Research Institute (US)
- Stanford University (US)
Publication Details
- Journal
- Investigative Ophthalmology & Visual Science
- Published
- 2024-12-18
- DOI
- https://doi.org/10.1167/iovs.65.14.32
- Citations
- 3
- Primary Topic
- Glaucoma and retinal disorders
- Type
- article
- Field-Weighted Citation Impact
- 1.62
Funders
- Glaucoma Research Foundation
- Research to Prevent Blindness
- BrightFocus Foundation
- Arrowhead Pharmaceuticals
- Aerie Pharmaceuticals
- National Institutes of Health
- Allergan