Toxicity Study of the Pharmacological Pair of Encapsulated Citrobacter freundii C115H Methionine γ-Lyase/Methiin
Acute and subchronic toxicity of the pharmacological pair based on encapsulated Citrobacter freundii C115H methionine γ-lyase enzyme/prodrug (methiin) was studied in female ICR mice. The drug showed a weak/moderate dose-dependent hepatotoxic effect. Most of the changes in liver morphology identified were insignificant or mild deviations from the norm. Long-term use of a single therapeutic dose per mouse of 1.5 U C. freundii C115H methionine γ-lyase @ (PEG−P(Asp)70/PLL70)-PIC-some/2 mg methiin led to a slight decrease in the weight of animals without obvious signs of intoxication. A quarter of the animals in this group had no deviations from the norm in liver morphology. No nephrotoxic effect was found in any study group.
Authors
- A. D. Lyfenko
- Anastasia S. Zemskaya (ORCID: https://orcid.org/0000-0002-0332-5105)
- Pavel N. Solyev (ORCID: https://orcid.org/0000-0002-5546-4975)
- Elena A. Morozova (ORCID: https://orcid.org/0000-0003-2922-2793)
- В. А. Казаков (ORCID: https://orcid.org/0000-0003-1641-2598)
- Vitalia V. Kulikova (ORCID: https://orcid.org/0000-0003-0618-1407)
- А. С. Чернов (ORCID: https://orcid.org/0000-0001-5371-1656)
- Svetlana V. Revtovich (ORCID: https://orcid.org/0000-0003-1594-9334)
- V. S. Koval (ORCID: https://orcid.org/0000-0002-1181-104X)
- Natalya V. Anufrieva (ORCID: https://orcid.org/0000-0002-6611-8840)
- G. B. Telegin
Institutions
- Engelhardt Institute of Molecular Biology (RU)
- Institute of Bioorganic Chemistry (RU)
Publication Details
- Journal
- Molecular Biology
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1134/s0026893324700560
- Citations
- 1
- Primary Topic
- Synthesis and Characterization of Heterocyclic Compounds
- Type
- article
- Field-Weighted Citation Impact
- 0.25