Sex differences in response to HBV vaccination in a cohort of health care workers

Globally, healthcare workers (HCWs) are at greater risk of contracting Hepatitis B virus (HBV) infection than the general population, due to their frequent contact with blood or body fluids. For this reason, WHO underlined the importance of HBV immunization for all HCWs. Although sex is now considered one of the key factors influencing the intensity and duration of the immune response to vaccines, sex-specific analysis of vaccine-induced anti-HBs antibodies is rarely conducted. Our study aimed to evaluate the anti-HBs antibodies' long-lasting protective titers in male and female HCWs to identify sex differences in the humoral response to the HBV vaccine. The study was conducted on 2106 HCWs (1636 females and 470 males) working at the Padova University Hospital, Italy. Our results report significantly higher anti-HBs levels in female HCWs than in males, particularly when considering HCWs for whom the elapsed time since vaccination is greater than 10 years, suggesting a greater persistence of anti-HBs in females. In conclusion, our findings could contribute to understanding differences in the antibody response to the HBV vaccine between male and female subjects and improving vaccination strategies for HCWs.