Comparative Characterization of Electrical and Panoramic Optical Mapping in Langendorff-Perfused Rabbit Hearts: From Sinus Rhythm to Fibrillation

Cardiovascular diseases (CVDs) are the leading causes of death globally.Comparative optical (gold standard) and electrical experimental setups have been developed to characterize the cardiac substrate and improve commercial medical equipment.We aim to characterize signals provided by a novel electrical and optical animal experimental setup across the entire heart, atrium, or ventricle and present differences between them using local activation time (LAT), cycle length (CL), frequency (F), heart rate (HR) and signal amplitude.New Zealand rabbit hearts were Langendorff-perfused, and electrical activity was acquired simultaneously with panoramic optical mapping and contact electrical mapping setup.Four rhythms were characterized: sinus with AVB (LA:162, V:42 bpm), atrial tachycardia with AVB (LA:463, V:73 bpm), atrial fibrillation with AVB (LA:428, V:0.4 bpm), and ventricular fibrillation (LA 223, V:490 bpm).The difference in electrical and optical LAT is smaller in SN-AVB (RA:12.24.3 ms) than in AF-AVB (RA:100.369.2ms).The difference in CL, F, and HR was less than 0.1 unit for SN and increases in AF up to 27 ms, 0.6 Hz, and 43 bpm, respectively.Optical results showed lower deviation values compared to electrical.Differences between electrical and optical results were presented since sinus to fibrillation.