Study on inheritance pattern and nutritional analysis of ATGRF2 transgenic 'Burgaltai' (Medicago varia Marthz)

Alfalfa (Medicago varia Marthz) 'Burgaltai' cultivar is one of the most important forages of its palatable and nutritional values. But still, national scientists had reported that there is lack of characteristic have been appeared in Mongolian cultivated Medicago sp., and it is the percentage of leaf in the yield is low (up to 45%) and it had decreased dramatically in development cycle. Over-expression of AtGRF2 resulted in larger leaves and cotyledons comparing to wild type. In this study, transgenic 'Burgaltai' with AtGRF2 gene had increased leaf area by 0.52 cm2 and leaves in percentage per bush up to 48.6%. The transgenic lines showed 3:1 segregation ratio in T1 progenies (χ2 = 0.04–0.42, p ≤ 0.52–0.84). Hygromycin antibiotic concentration is 50 mg/l for selective media. According to the feed evaluation, some chemical composition increased in transgenic 'Burgaltai' except total fat.