Novel approach using automated target enrichment enables culture-independent accurate whole-genome sequencing of <i>Neisseria gonorrhoeae</i> directly from clinical urogenital and extragenital specimens
We show that custom SureSelectXTHS target enrichment automated on the Magnis NGS Prep System, followed by Illumina sequencing, enables culture-independent genome-based surveillance of N. gonorrhoeae AMR and molecular epidemiology. This novel methodological advancement provides an efficient and accurate WGS of N. gonorrhoeae directly from clinical urogenital and extragenital NAAT specimens.
Authors
- Daniel Golparian (ORCID: https://orcid.org/0000-0002-0688-2521)
- Ellionor Rapp
- Magnus Unemo (ORCID: https://orcid.org/0000-0003-1710-2081)
- Johanna Hasmats
Institutions
- Örebro University (SE)
- Örebro University Hospital (SE)
- Agilent Technologies (Sweden) (SE)
- University College London (GB)
Publication Details
- Journal
- Journal of Antimicrobial Chemotherapy
- Published
- 2024-12-16
- DOI
- https://doi.org/10.1093/jac/dkae446
- Citations
- 4
- Primary Topic
- Bacterial Infections and Vaccines
- Type
- article
- Field-Weighted Citation Impact
- 1.38