Comparative study of anti-staphylococcal efficacy: traditional homeopathic dilutions vs. nano-enhanced Rhus Tox formulations

Abstract Background Staphylococcus aureus is a leading cause of death, especially among the elderly. This bacterium produces several surface membrane proteins, with staphylococcal protein A (SpA) being particularly important. Despite its prevalence, there are no targeted treatments available for geriatric patients. In homeopathy, Rhus toxicodendron (RT) is frequently used in various dilutions-Mother Tincture (MT), 6CH, 30CH, and 200CH- for conditions like skin infections, soft tissue disorders, and joint ailments. Methods In this study, we evaluated the effects of Rhus toxicodendron (RT) at different concentrations on Staphylococcus aureus through bacterial plate cultures and compared the outcomes with Nano-RT. Notably, Nano-RT MT is not commercially available. This research is the first to showcase both the efficacy and biosafety of the innovative nano-ZnO RT MT homeopathic formulation. Results This study examined the inhibitory effects of Rhus toxicodendron in its Mother Tincture (MT) form and in 6CH, 30CH, and 200CH dilutions against Staphylococcus aureus . Nano-ZnO was synthesized from Rhus toxicodendron MT and combined to create Nano-MT. Both Rhus Tox MT and Nano-RT MT demonstrated significant inhibition of Staphylococcus aureus within 24 h of application. Conclusions Rhus Tox MT and Nano-RT MT present promising new options for treating Staphylococcus aureus infections in elderly patients. Furthermore, studies have demonstrated that Nano-RT MT is completely biologically safe in mice.