Obtaining Haploid Plants Via Anther Culture in Some Eggplant Varieties

In plant breeding studies, anther culture is used to breed hybrid varieties, to transform tetraplaid plants that can be obtained through interspecific somatic hybridization into fertile dihaploid plants, and in recent years, to obtain diploid in protoplast fusion of haploid embryogenic calli obtained from anther culture. The frequency of obtaining haploid plants in anther culture depends on many factors such as the variety, the season in which it was taken and the use of suitable media for regeneration. In this study, it was aimed to determine the appropriate regeneration environment in the anther culture of Aydın Siyahı and Kemer eggplant varieties, which are widely grown in our country. In the anther culture study, C (callus), R (regeneration) and V3 media recommended by Dumas de Valux et al. (1982) were used as nutrient media. However, as C (callus) medium, Dumas de Vaulx et al. (1982) 3 different doses of 2,4 D, Kinetin and sucrose of the C medium reported were tested. The highest plant yield (36.4%) of the Aydin Siyahı variety was obtained from the modified Dumas de Vaulx et al. (1982), from 1 mg/l 2.4-D +1 mg/l Kinetin + 120 g/l sucrose medium, the highest plant yield (33.8%) of the Kemer variety was reported by Dumas de Vaulx et al. (1982), obtained from 5 mg/l 2.4-D + 5 mg/l Kinetin + 120 g/l sucrose medium. Of the 79 plants obtained from anther culture and whose ploidy level was examined, 60 were determined as haploid, 13 as diploid, 4 as triploid, 1 as tetraploid and 1 as mixoploid.