Polyhydroxyalkanes from <i>Pachysandra terminalis</i> Sieb. et Zucc

Four polyhydroxyalkanes were isolated from the CH₂Cl₂ part of <i>Pachysandra terminalis</i>, including two new ones, (2<i>S</i>,3<i>E</i>)-3-isopropylpent-3-ene-1,2,5-triol (<b>1</b>) and (4<i>R</i>,2<i>E</i>)-4,5-dihydroxy-2,3-dimethylpent-2-enoic acid (<b>2</b>); as well as one new natural product, 3-methylbut-3-ene-1,2-diol (<b>3</b>) and one known compound, 4-methyl-3-methylenepentane-1,2-diol (<b>4</b>). The structures were determined by physicochemical properties and spectroscopic methods including 1D, 2D NMR, IR, HR-ESI-MS, and ECD data. The cytotoxic activity of compounds against the human cancer lines A549, H460, and HepG2 cell lines of the isolated compounds was evaluated by the CCK8 method. However, compounds did not show significant activity.