Rapid diagnostic testing method to detect ROB β-lactamase gene in Neisseria meningitidis
bla<sub>ROB-1</sub> is the only widely found β-lactamase in Neisseria meningitidis, and its presence is on the rise. To enhance our bacterial meningitis testing procedure, we clinically validated a real-time PCR assay to rapidly detect the bla<sub>ROB</sub> gene and predict drug resistance in Neisseria meningitidis. A screen of 101 clinical isolates and 37 clinical specimens of blood and cerebrospinal fluid received between January 2018 and June 2024 found 8 isolates and 2 cerebrospinal fluid specimens that were positive for bla<sub>ROB</sub>.
Authors
- Geetha Nattanmai
- Kimberlee A. Musser (ORCID: https://orcid.org/0000-0003-1922-5405)
- Katharina Wahl
- Anna Kidney
- Kara Mitchell
- Linnell Randall
- Andrew C. Peifer
- Edward D. Owuor
- Sherly Jose
- Erin Klingbeil
Institutions
- New York State Department of Health (US)
- Wadsworth Center (US)
Publication Details
- Journal
- Molecular and Cellular Probes
- Published
- 2024-12-19
- DOI
- https://doi.org/10.1016/j.mcp.2024.102000
- Citations
- 2
- Primary Topic
- Bacterial Infections and Vaccines
- Type
- article
- Field-Weighted Citation Impact
- 0.69
Funders
- Centers for Disease Control and Prevention