Analytical Method development and validation of Amlodipine Besilate and Perindopril Erubamine by using Reverse Phase Ultra Performance Liquid Chromatography

A simple, Accurate, precise method was developed for the simultaneous estimation of the Amlodipine Besilate and Perindopril Erubamine in dosage form.Chromatogram was run through ACQUITY UPLC CSH C18 Column, 1.7 m, 2.1 mm X 150 mm.Mobile phase containing Acetonitrile: Methanol: of 0.7% v/v of triethylamine in water taken in the ratio 15:35:50 was pumped through column at a flow rate of 0.4 ml/min.Buffer used in this method was 0.01N KH2PO4.Temperature was maintained at 30C.Optimized wavelength selected was 237 nm.Retention time of Amlodipine Besilate and Perindopril Erubamine were found to be 0.909 min and 1.398.%RSD of the Amlodipine Besilate and Perindopril Erubamine were and found to be 0.7 and 0.6 respectively.%Recovery was obtained as 100.13% and 100.10% for Amlodipine Besilate and Perindopril Erubamine respectively.LOD, LOQ values obtained from regression equations of Amlodipine Besilate and Perindopril Erubamine were 0.19, 0.56 and 0.04, 0.12 respectively.Regression equation of Amlodipine Besilate is y = 70199x + 13831, and y = 710668x + 2772.6 of Perindopril Erubamine.Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.