The <i>Crinum amabile’s </i>Aerial Organs Fatty Acids, Steroids, Flavonoids, Study of Their Antioxidant and Anti-Inflammatory Activities

The <i>Crinum amabile</i> is a plant whose alkaloids medicinal virtues are well discussed in bibliographies. However, specific studigges on the molecules present in the aerial organs of <i>Crinum amabile</i>, as well as their virtues as antioxidant and anti-inflammatory are still few and far between. This led us to undertake studies to determine and quantify the fatty acid molecules, then the steroid molecules, then to determine the flavonoid molecules in the various organs systems of the <i>Crinum amabile </i>and also to determine their alkaloids quantities. The method used to extract the various molecules present in the different organs of the <i>Crinum amabile</i> is the esterification with citric acid molecules to get citric acid ester solutions of their bioactive molecules. The fatty acid molecules were extracted by soxhlet using hexane and identified by Phase Gas Chromatography; so, the fatty acid molecules present in the various organs of <i>Crinum amabile</i> are myristic, palmitic, palmitoleic, stearic, oleic, linoleic, linolenic and arachidic acids whose antioxidant with anti-inflammatory chemical properties are very interesting. HPLC was used for steroids and flavonoids quantifications after transesterification and extraction of the active molecules in citric acid esters from the various aerial organs of the <i>Crinum amabile</i>. The analysis showed that the various aerial organs or organ systems of the <i>Crinum amabile</i> contained flavonoids and steroids that are respectively eriocitrin, hesperidin, neohesperidin, isorhoifolin, rhoifolin and betulinic acid, ursolic acid, betuline, lupeol whose antioxidant with anti-inflammatory chemical properties are also very interesting. These different bioactive molecules extracted into citric acid ester solutions of the various organs or organ systems of the <i>Crinum amabile</i> were subjected to dpph antioxidant test and NO scavenging anti-inflammatory test and the results were very promising, given that the organ system « anther-stamen fillet-pistil-perianth had the most effective IC50 for both antioxidant and anti-inflammatory activity, followed by organ system « stem-receptacle-leaf » and finally the organ « petal ». In addition, kinetic studies were carried out during the esterification reactions with citric acid of the various <i>Crinum amabile</i> organs, making it possible to deduce their kinetic constants and subsequently to show the effect of the rigidity of their structures and porosities on their antioxidant and anti-inflammatory activities at the level of their citric acid ester solutions. Determination of alkene content in the various <i>Crinum amabile</i> organ system stock solutions used in the anti-inflammatory tests confirmed and reported their effective role as carriers of reactive and bioactive molecules.