STUDY ON SUBCLINICAL ENDOMETRITIS IN CATTLE OF KATHMANDU VALLEY BY USING WHITE SIDE TEST (WST)

The current investigation aimed to identify the different levels of nonspecific bacterial infection in the genitalia of repeat-breeding cattle of Kathmandu District using WST. A total of 307 crossbred Holstein Friesian cows were considered for this purpose, out of which 246 repeat-breeding cattle were under treatment and 61 cattle with normal cycle (control group) were artificially inseminated at their first service. Cervical mucus samples were collected 8 to 12 hours after the first signs of behavioral estrus, and subjected to a white side test (WST) and bacteriological examination. From the results of WST, it has been inferred that only 9(14.75%) animals in the control group were positive and the remaining 52(85.24%) were negative. However, the majority of repeat-breeding animals were positive 190 (77.22%)) and only 56 (22.76%) of animals were found negative. Microbiological examination of 190 samples revealed E.coli 54(28.421%), Staphylococcus spp. 39(20.526%), Streptococcus spp. 26(13.684%), Corynebacterium spp. 10(5.263%), Pseudomonas spp. 7(3.684%), Klebsiella spp. 9(4.736%), and mix bacterial growth 44(23.157%). The remaining 56 repeat-breeding cattle were negative with WST, possibly due to the absence of bacteria. Other causes may be viruses, placental retention, ovarian cysts, dystocia, etc. The findings of the present study divulge that WST may be utilized in the field to detect subclinical endometritis caused by bacteria.