Biosynthesis and Characterization of Nanoparticles from Cheilocostus speciosus Rhizome Plant Extracts

This study was aimed to analyze the anti-cancer activity of silver nanoparticles (AgNPs) synthesized using five different solvent extracts from the rhizome of Cheilocostus speciosus.Synergistic five different solvent extract of rhizome of Cheilocostus speciosus was used to biosynthesis of AgNPs.Characterization of AgNPs was performed using UV-visible spectroscopy, DLS and SEM analyses.Anti-cancer activity of AgNPs against SK-MEL-28 cells was tested using MTT assay.UV-Visible spectroscopy analysis indicated the surface plasmon resonance (SPR) sharp peak at 350-430 nm wavelength that corresponds to the production of AgNPs.SEM analysis showed that AgNPs are in a spherical shape with a size of 42-61 nm.Anti-cancer study reveals that AgNPs had shown cytotoxicity against SK-MEL-28 cells at the concentrations ranged from 25 to 500 g/mL and IC50 at 150.8 g/mL.This study concludes that AgNPs synthesized using rhizome of of Cheilocostus speciosus possesses potential anti-cancer activity.