O003 Higher central circadian temperature amplitude is associated with greater metabolite rhythmicity in humans

Abstract Introduction Robust circadian rhythms are essential for optimal health. The central circadian clock controls temperature rhythms, which are known to organize the timing of peripheral circadian rhythms in rodents. In humans, however, it is unknown whether temperature rhythms relate to the organization of circadian rhythms throughout the body. Methods We assessed core body temperature amplitude and the rhythmicity of 929 blood plasma metabolites across a 40-hour constant routine protocol, controlling for behavioral and environmental factors that mask endogenous temperature rhythms, in 23 healthy individuals (mean [±SD] age = 25.4±5.7 years, 5 females). Valid core body temperature data were available in 17/23 (mean [±SD] age = 25.6±6.3 years, 1 female). Results Individuals with higher core body temperature amplitude had a greater number of metabolites exhibiting circadian rhythms (R²=0.37, p=.009). Higher core body temperature amplitude was also associated with less variability in the free-fitted periods of metabolite rhythms within an individual (R²=0.47, p=.002). Discussion These findings indicate that a more robust central circadian clock is associated with greater organization of circadian metabolite rhythms in humans. Metabolite rhythms may therefore provide a window into the strength of the central circadian clock.