Auxin-dependent post-translational regulation of MONOPTEROS in the Arabidopsis root
Auxin plays a pivotal role in plant development by activating AUXIN RESPONSE FACTORs (ARFs). Under low auxin levels, ARF activity is inhibited by interacting with Aux/IAAs. Aux/IAAs are degraded when the cellular auxin concentration increases, causing the release of ARF inhibition. Here, we show that levels of the ARF5/MONOPTEROS (MP) protein are regulated in a cell-type-specific and isoform-dependent manner. We find that the stability of MP isoforms is differentially controlled depending on the auxin level. The canonical MP isoform is degraded by the proteasome in root tissues with low auxin levels. While auxin sharpens the MP localization domain in roots, it does not do so in ovules or embryos. Our research highlights a mechanism for providing spatial control of auxin signaling capacity. Together with recent advances in understanding the tissue-specific expression and post-transcriptional modification of auxin signaling components, these results provide insights into understanding how auxin can elicit so many distinct responses.
Authors
- Alex Cavalleri (ORCID: https://orcid.org/0000-0003-4929-222X)
- Jekaterina Truskina (ORCID: https://orcid.org/0000-0001-7428-7356)
- José M. Muiño (ORCID: https://orcid.org/0000-0002-6403-7262)
- Elina Chrysanthou (ORCID: https://orcid.org/0000-0002-6298-9243)
- Mara Cucinotta (ORCID: https://orcid.org/0000-0002-5410-9912)
- Teva Vernoux (ORCID: https://orcid.org/0000-0002-8257-4088)
- Kerstin Kaufmann (ORCID: https://orcid.org/0000-0001-7960-6256)
- Rahul Bhosale (ORCID: https://orcid.org/0000-0001-6515-4922)
- Lucia Colombo (ORCID: https://orcid.org/0000-0001-8415-1399)
- Etienne Farcot (ORCID: https://orcid.org/0000-0002-0742-3028)
- Martin M. Kater (ORCID: https://orcid.org/0000-0003-1155-2575)
- Anthony Bishopp (ORCID: https://orcid.org/0000-0003-2962-9542)
- Chiara Astori (ORCID: https://orcid.org/0009-0009-4244-1916)
- Dolf Weijers (ORCID: https://orcid.org/0000-0003-4378-141X)
- Xiaocai Xu (ORCID: https://orcid.org/0000-0002-2972-4251)
- Malcolm J. Bennett (ORCID: https://orcid.org/0000-0003-0475-390X)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- École Normale Supérieure de Lyon (FR)
- Centre National de la Recherche Scientifique (FR)
- University of Nottingham (GB)
- University of Milan (IT)
- Cal Poly Humboldt (US)
- Humboldt-Universität zu Berlin (DE)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Laboratoire Reproduction et Développement des Plantes (FR)
- International Crops Research Institute for the Semi-Arid Tropics (ML)
- Wageningen University & Research (NL)
Publication Details
- Journal
- Cell Reports
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.celrep.2024.115083
- Citations
- 12
- Primary Topic
- Plant Molecular Biology Research
- Type
- article
- Field-Weighted Citation Impact
- 7.20
Funders
- Royal Society
- Deutsche Forschungsgemeinschaft
- Agence Nationale de la Recherche
- Ministero dell’Istruzione, dell’Università e della Ricerca
- Emergence de Jeune Equipe INRA
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
- Biotechnology and Biological Sciences Research Council
- European Research Council