Structural Characterization of Protein Complexes Using Native Mass Spectrometry

Native mass spectrometry (native MS) is an analytical technique to determine molecular masses of proteins and protein complexes without crosslinking, which provides the composition and stoichiometry for their structural characterization. We have used mass spectrometry for structural biology, including native MS, to characterize proteins and protein complexes. In this paper, I present a brief overview of native MS. In addition, I highlight the structural characterization of intrinsically disordered protein complexes using ion mobility mass spectrometry and the importance of stoichiometry using native MS including the methodology of native MS in the presence of nonvolatile buffer among my researches.