Study on the Possibility of Aflatoxin Contamination in Fermentation Starter

This study explored the possibility of Aspergillus flavus contamination and toxin production during the fermentation of starter. Aspergillus flavus isolated from natural fermentation was selected as the research object, and mixed with Transocephalia racemosa and Mucor circinelloides to establish starter. Toxin production and toxin level of Aspergillus flavus in starter were analyzed by PCR, HPLC, competitive ELISAand mouse experiment. The results showed that the isolated Aspergillus flavus strain contained aflR, omt-1 and ver-1 gene fragments; HPLC chromatogram showed that this strain mainly produced AFB1 and the concentration was 16.8µg/kg; Aspergillus flavus grew well on the drug block under construction, completely covered the drug block surface after 36h, and had strong toxin production ability. When mixed with Transverse Rhizopus racemosus and Mucor circinellus to establish starter, the growth and toxin production ability of Aspergillus flavus were inhibited. Afteroral administration oftwo kinds of AFB1-containing extractsto mice, the liver tissue was obviously damaged, and Jianqu did not show inhibitory effect on the toxicity of AFB1. This study provides a reference for strengthening quality control and preventing pollution in the fermentation process of Jianqu.