Beyond Dimerization: Harnessing Tetrameric Coiled‐Coils for Nanostructure Assembly
Versatile DNA and polypeptide-based structures have been designed based on complementary modules. However, polypeptides can also form higher oligomeric states. We investigated the introduction of tetrameric modules as a substitute for coiled-coil dimerization units used in previous modular nanostructures. Tetramerizing helical bundles can run in parallel or antiparallel orientation, expanding the number of topological solutions for modular nanostructures. Furthermore, this strategy facilitates the construction of nanostructures from two identical polypeptide chains. Importantly, tetrameric modules substantially stabilized protein nanostructures against air-water interface denaturation, enabling the determination of the first cryo-electron microscopy three-dimensional structure of a coiled-coil-based nanostructure, confirming the designed agreement of the modules forming a tetrahedral cage.
Authors
- Sara Vidmar (ORCID: https://orcid.org/0000-0002-9974-1858)
- Florian Beck (ORCID: https://orcid.org/0000-0003-3183-2953)
- Tamara Šmidlehner (ORCID: https://orcid.org/0000-0001-5097-9606)
- Ajasja Ljubetič (ORCID: https://orcid.org/0000-0002-2171-1579)
- Roman Jerala (ORCID: https://orcid.org/0000-0002-6337-5251)
- Jana Aupič (ORCID: https://orcid.org/0000-0001-6246-962X)
- Philipp S. Erdmann (ORCID: https://orcid.org/0000-0001-8289-9133)
- Fei Xiao (ORCID: https://orcid.org/0000-0001-5262-2475)
- Žiga Strmšek (ORCID: https://orcid.org/0000-0001-9226-3497)
- Guang Hu (ORCID: https://orcid.org/0000-0002-8754-1541)
- Chuan Liu (ORCID: https://orcid.org/0000-0003-2441-7399)
Institutions
- University of Ljubljana (SI)
- Soochow University (CN)
- Human Technopole (IT)
- EN-FIST Centre of Excellence (Slovenia) (SI)
- Max Planck Institute of Biochemistry (DE)
- National Institute of Chemistry (SI)
Publication Details
- Journal
- Angewandte Chemie International Edition
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1002/anie.202422075
- Citations
- 2
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- European Commission
- Javna Agencija za Raziskovalno Dejavnost RS
- H2020 Future and Emerging Technologies
- HORIZON EUROPE European Research Council