Marine Actinomycete Streptomyces rubrogriseus Derived Bioactives are Effective Against Clinical Strains of MRSA

Introduction: Natural microbial communities interact to develop mutualistic relationships creating a competitive environment stimulating secondary metabolite production which may be developed as a potential drug against drug-resistant bacteria. The current research delves into the possibility of co-culturing marine actinomycetes to elevate the production of novel bioactive compounds with enhanced antimicrobial activity against Methicillin-­resistant Staphylococcus aureus (MRSA). Method:. Actinomyocyte was isolated from marine sediments, identified as Streptomyces rubrogresius was cocultured with S. aureus. The bioactive compounds were extracted and evaluated for activity against MRSA variants. The extracts exhibiting significant activity were further characterized using GC-MS. Results: The study demonstrated a significant increase in the production of bioactive compounds in co-culture compared to the monoculture. When tested against MRSA strains, inhibition zones obtained from ethyl acetate extracts of co-culture (40mm) revealed substantial differences when compared with that of monocultures (MIC: 10 μg/mL). GC-MS analysis identified unique chemical compositions and potential synergistic outcomes in the coculture rather than monoculture. Conclusions: The findings from this study are of paramount importance as they aid in the discovery of novel antibiotics effective against MRSA.