Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC
This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single-cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor-draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single-cell suspensions can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, etc. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.
Authors
- Claudia Zelle‐Rieser (ORCID: https://orcid.org/0000-0002-0752-1795)
- William W. Agace (ORCID: https://orcid.org/0000-0003-3823-5772)
- Lukas Heger (ORCID: https://orcid.org/0000-0001-5591-2187)
- Charles‐Antoine Dutertre (ORCID: https://orcid.org/0000-0001-7950-3652)
- Yael Horev
- Helen Strandt (ORCID: https://orcid.org/0000-0002-1488-1633)
- Vinitha Kandiah
- Thomas M. Fenton (ORCID: https://orcid.org/0000-0002-6200-6403)
- Ruth Lubin (ORCID: https://orcid.org/0000-0003-4077-7968)
- Oded Heyman
- Joyce Bakker
- Christoph H. Tripp (ORCID: https://orcid.org/0000-0001-5502-7241)
- Gabriel Mizraji (ORCID: https://orcid.org/0000-0003-2692-1732)
- Florent Ginhoux (ORCID: https://orcid.org/0000-0002-2857-7755)
- Tanja D. de Gruijl (ORCID: https://orcid.org/0000-0003-1464-8978)
- Florian Hornsteiner
- Asaf Wilensky (ORCID: https://orcid.org/0000-0001-9002-6041)
- Kim van Pul
- Simon Yona (ORCID: https://orcid.org/0000-0002-3984-2008)
- Sieghart Sopper (ORCID: https://orcid.org/0000-0003-2265-1974)
- Diana Dudziak (ORCID: https://orcid.org/0000-0001-9358-134X)
- Rieneke van de Ven (ORCID: https://orcid.org/0000-0002-4230-8383)
- Elisa C. Toffoli (ORCID: https://orcid.org/0000-0003-0695-6479)
- Patrizia Stoitzner (ORCID: https://orcid.org/0000-0002-8488-6704)
- Martina Sykora (ORCID: https://orcid.org/0000-0002-0937-1137)
- Regine J. Dress (ORCID: https://orcid.org/0000-0001-8785-5471)
- Natasa Prokopi (ORCID: https://orcid.org/0000-0002-6009-9335)
- Marieke F. Fransen (ORCID: https://orcid.org/0000-0003-3036-8618)
- Or Saar
- Paz Kles
Institutions
- Agency for Science, Technology and Research (SG)
- University of Copenhagen (DK)
- Innsbruck Medical University (AT)
- Inserm (FR)
- Universität Hamburg (DE)
- Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
- Shanghai Jiao Tong University (CN)
- Lund University (SE)
- Universität Innsbruck (AT)
- Hebrew University of Jerusalem (IL)
- Hadassah Medical Center (IL)
- Singapore Immunology Network (SG)
- Institut Gustave Roussy (FR)
- Universitätsklinikum Erlangen (DE)
- SingHealth Duke-NUS Academic Medical Centre (SG)
- Tyrolean Cancer Research Institute (AT)
- University Medical Center Hamburg-Eppendorf (DE)
- Oncode Institute (NL)
- LEO Foundation (DK)
- Jena University Hospital (DE)
- Amsterdam University Medical Centers (NL)
- Cancer Center Amsterdam (NL)
- Friedrich Schiller University Jena (DE)
- University of Glasgow (GB)
- Vrije Universiteit Amsterdam (NL)
Publication Details
- Journal
- European Journal of Immunology
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1002/eji.202250325
- Citations
- 4
- Primary Topic
- T-cell and B-cell Immunology
- Type
- article
- Field-Weighted Citation Impact
- 0.96
Funders
- Österreichischen Akademie der Wissenschaften
- Lundbeckfonden
- Israel Science Foundation
- Österreichische Forschungsförderungsgesellschaft
- Medicinska Forskningsrådet