Three Spectrophotometric Methods Validated for Handling of Overlapped Spectra: Application for Determination Propranolol Hydrochloride and Hydrochlorothiazide in Dosage Forms and spiked biological fluids

This research evaluates a binary mixture of Propranolol hydrochloride (PRO) and Hydrochlorothiazide (HCT) in pure powder form, dosage and spiked biological fluids via three spectrophotometric approaches which are Derivative ratio (DD1), Ratio difference (RD) and Area under the curve (AUC). Zero order spectrum (D0) of binary mixture of (PRO) and (HCT) has revealed significant interfering with nominal extension furthermore lack of isoabsorbitive points which is considered a challenge in spectral analysis. In other respects, esteeming presented combination in spiked biological fluids in literary works had some limitations. These approaches were facilitated to identify paired mixture spectrophotometrically nevermore preceding segregation as a consequence resolved the overly outcome of their zero order spectra. Linearity range was (2.0-9.0), (1.5-9.0) µg/mL for Propranolol hydrochloride and Hydrochlorothiazide appropriately. This linearity range permits to reveal minimum toxic concentrations (MTC) in spiked biological fluids so this project raises enforcements of smart spectrophotometric feasible applications in quality control and toxicological laboratories. The three approaches were proved by utilizing ICH confines and were established to be fruitful in estimating the existence of Propranolol hydrochloride and Hydrochlorothiazide in dosage forms and spiked biological fluids. The outcomes of the suggested approaches were arithmetically compared to USP pharmacopeia approach, along with never main deviation was perceived.