Preparation, Characterisation and Antifungal activity of Bi-Metallic Nanoparticles prepared with Fresh Extract of Aconitum heterophyllum leaves

A Bi-Metallic Nanoparticles of the green approach for the biosynthesized of well – stabilized nanoparticles, the synthesis of nanoparticles using plant extracts has gained considerable attention in the field of nanotechnology since it is easy, simple, and cost effective and does not make use of toxic chemicals. In this work, gold/silver (Au /Ag) bimetallic nanoparticles were synthesized using the Aconitum heterophyllum leaf extract. The advantages of using plant and plant-derived materials for biosynthesis of Bi-Metallic Nanoparticles have attract the interest of researchers due to its better mechanisms in successful formation of Bi-Metallic Nanoparticles. The metallic structurer were characterized using various methods. The metal nanoparticles were characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy, zeta potential, XRD, AFM, TEM, STEM, EDX analysis. The Minimum inhibitory concentration of the nanoparticles on various microorganisms (S. aureus, E.coli, P. aeruginosa, B. subtilis and C. albicans) was determined along with its anti-biofilm and photocatalytic activity. The bimetallic nanoparticles exhibited better and enhanced activity in inhibiting the microorganisms as well as the anti-biofilm activity in comparison to gold nanoparticles. However, the photocatalytic activity was found to be better in the gold nanoparticles as compared to the bimetallic nanoparticles.