CHANGES IN THE INTENSITY OF PROTEIN AND LIPID PEROXIDATION IN MUSCLE AND ADIPOSE TISSUES, PRO-/ANTIOXIDANT POTENTIAL OF BLOOD SERUM IN RATS WITH ZINC AND IODINE DEFICIENCY AND HIGH-CALORIE DIET

The aim of the study – to determine the intensity of oxidative modification processes ofproteins and lipid peroxidation in the blood serum, muscle and adipose tissue, and theantioxidant potential of the blood serum in rats with iodine and zinc deficiency, highcarbohydrate and high-fat diets.Materials and methods. The study was performed on outbred white mature rats (males) feda standard vivarium diet, zinc- and iodine- deficient, high-fructose and high-fat diets for 8weeks. The content of products of oxidative modification of proteins and lipoperoxidation(diene conjugates and products that react to thiobarbituric acid) was investigated in theblood serum, thigh and lower leg muscle homogenates and visceral fat.Results. The activation of oxidative modification procesees of proteins in skeletal musclesand blood serum (most of all under high-fat diet), in adipose tissue (most intensively inanimals with zinc and iodine deficiency) was found compared to the control. A significantincrease in lipid peroxidation products was detected in blood serum (in the context ofa high-fat diet), in muscles (under iodine deprivation), and in visceral fat (especially underhigh-fat diet) compared with the data from animals on a standard diet and drinking regimenin vivarium. Such disorders of oxidative processes were observed against the background of a decrease in superoxide dismutase activity and catalase activation, an increase in serumceruloplasmin content, regardless of diet. The activity of glutathione peroxidase increasedunder conditions of zinc deficiency and high-calorie feeding, but was suppressed against thebackground of iodine deprivation. The iron saturation of transferrin exceeded the controlvalues against the background of zinc and iodine deficiency and high- fat feeding.Conclusions. Under conditions of zinc and iodine deficiency, high-calorie feeding,oxidative processes in skeletal muscles and visceral fat are activated against thebackground of antioxidant imbalance in blood serum (especially when animals are ona high-fat diet). The highest intensity of oxidative modification of proteins and lipidperoxidation in skeletal muscles was observed under high-fat diet conditions, and invisceral fat – against the background of zinc and iodine deficiency.