190P Ex vivo modeling of precision immuno-oncology responses in lung cancer
Naaa + dendritic cells (DCs), among others.TLS also enriched for certain clones in TCR/ BCR repertoires.Conclusions: Through photocatalytic covalent labeling with biotin, we achieved image-guided labeling of live TLS cells for scRNA-seq and identified TLS-enriched cells with potential functions in antigen presentation, cytotoxicity and antibody secretion.We anticipate detecting changes in TLS signatures and functions following PI3K drug treatment, enabling a comprehensive analysis of TLS.Moreover, we plan to refine our methodology to expand the scope of application in other crucial biomedical research areas.
Authors
- Kimmo Peltonen
- J. Klievink
- Shadi Jansouz
- Mikko Rönty
- Jari Räsänen (ORCID: https://orcid.org/0000-0002-7826-532X)
- Juhani Smolander
- Eva Sutinen (ORCID: https://orcid.org/0000-0002-9169-7178)
- Pipsa Saharinen (ORCID: https://orcid.org/0000-0003-2652-0584)
- Laura Lahtinen (ORCID: https://orcid.org/0000-0001-7559-2168)
- Mari Ainola (ORCID: https://orcid.org/0000-0003-2853-5851)
- Ilkka Ilonen (ORCID: https://orcid.org/0000-0002-8149-3550)
- Hanna Laitinen (ORCID: https://orcid.org/0009-0008-7505-5512)
- S. Mustjoki
- Elli Narvi (ORCID: https://orcid.org/0000-0002-1468-5587)
- Heidi M. Haikala (ORCID: https://orcid.org/0000-0002-8465-8568)
- Bassel Alsaed
- Elina Jokinen (ORCID: https://orcid.org/0000-0002-8345-1629)
- Li Li (ORCID: https://orcid.org/0009-0003-4636-9487)
- Anil K. Thotakura
- Nina Bobik
Institutions
- University of Helsinki (FI)
- Helsinki University Hospital (FI)
- Orion Corporation (Finland) (FI)
Publication Details
- Journal
- Immuno-Oncology Technology
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.iotech.2024.100943
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00