Amycolatopsis heterodermiae sp. nov. and Actinacidiphila polyblastidii sp. nov., two new actinobacteria isolated from foliose lichens

Two novel actinomycete strains, designated as V23-08 T and V4-01 T , were isolated from a foliose lichen collected in Doi Inthanon National Park, Chiang Mai Province, Thailand. Strain V23-08 T showed the highest similarity (98.40%) to Amycolatopsis tolypomycina DSM 44544 T . Similarly, strain V4-01 T showed the highest 16S rRNA gene similarity relatedness (98.89%) to Actinacidiphila acidipaludis PLK6-54 T . Chemotaxonomic analysis revealed distinct characteristics for each strain. The isomers of diaminopimelic acid were identified as meso -DAP in strain V23-08 T and ll -DAP in strain V4-01 T . The predominant menaquinones were MK-9(H 2 ) for strain V23-08 T and MK-9(H 6 ) and MK-9(H 8 ) for strain V4-01 T . Strain V23-08 T contained mannose, glucose, galactose and arabinose as whole-cell sugars, with iso -C 16:0 as the major fatty acid. The polar lipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipids, unidentified phospholipids and unidentified lipids. In contrast, strain V4-01 T exhibited glucose and galactose as whole-cell sugars, with iso -C 16:0 , anteiso -C 16:0 and C 16:0 as major fatty acids, and a polar lipid composition of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The taxonomic data, supported by digital DNA–DNA hybridization and average nucleotide identity analyses, confirm that strains V23-08 T and V4-01 T represent novel species within the genera Amycolatopsis and Actinacidiphila , respectively. Based on these findings, the names Amycolatopsis heterodermiae sp. nov. (strain V23-08 T : TBRC 16208 T and NBRC 115837 T ) and Actinacidiphila polyblastidii sp. nov. (strain V4-01 T : TBRC 16209 T and NBRC 115865 T ) are proposed.