Optimization of Binding Buffer Composition (Polyethylene Glycol, Sodium Chloride and pH) for Extraction of DNA from Biological Fluids Using Polyethyleneimine Functionalized Iron Oxide Nanoparticle-Based Method
The findings highlighted the critical role of binding buffer composition in maximizing DNA recovery. The use of optimized PEI-IONPs provided a rapid and efficient method for DNA extraction, supporting its potential for applications in scientific and clinical research. Future studies should explore the robustness of these optimized conditions across diverse biological fluids and extraction settings.
Authors
- Gaurav Kaushik (ORCID: https://orcid.org/0000-0001-6743-7811)
- Richa Vashishtha
- Chaitenya Verma (ORCID: https://orcid.org/0000-0001-5723-6346)
- Imran Khan (ORCID: https://orcid.org/0000-0003-4750-8189)
- Vinay Kumar (ORCID: https://orcid.org/0000-0002-6751-5741)
Institutions
- All India Institute of Medical Sciences (IN)
- Sharda University (IN)
- Penn State Milton S. Hershey Medical Center (US)
Publication Details
- Journal
- Nanotechnology Science and Applications
- Published
- 2024-12-01
- DOI
- https://doi.org/10.2147/nsa.s494613
- Citations
- 4
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Sharda University