Evaluation of the cancer stem cell characteristics of acquired resistant BT549 GEM100nM breast cancer cells

Stem cells playing a vital role in cancer biology have drawn more attention in recent years. Many dense cancers, involving the breast, brain, colon, liver, and pancreas, have been found to be associated with cancer stem cells (CSCs). The objective of this research is to evaluate the cancer stem markers of an attained resistant BT549 GEM100nM breast cancer cell line. Fluorescence-activated cell sorting (FASC) results showed that the resistant cell lines contained higher levels of embryonic markers (Nanog, Oct 4, and Sox2) and cancer stem cell markers (ALDH and CD 133) than the parental wild-type cell (BT549). It is thought that these CSC indicators are pivotal for cancer genesis, relapse, spread and treatment resistance.