A Reliable Voltammetric Method Utilizing the Effect of an Anionic Surfactant for the Determination of Leuprolide

In this study, the rapid, simple and sensitive voltammetric method has been developed for the quantification of leuprolide acetate in the presence of anionic surfactant on a glassy carbon electrode. The effect of surfactant types, pH of the medium, scan rate on the voltammetric response of leuprolide acetate were evaluated. The oxidation peak currents of leuprolide acetate were enhanced in the presence of 8.0 x 10-5 M sodium dodecyl sulfate. The monomer structure of sodium dodecyl sulfate can be attracted to amino moieties in drug structure via the electrostatic interaction. The voltammetric behavior of leuprolide acetate exhibited irreversible and diffusion-adsorption mix controlled processes by cyclic voltammetry. Under optimized conditions, the proposed method exhibits linearity in the concentration range of 3.64 x 10-9 - 2.00 x 10-7 M with a nano-level detection limit of 4.70 x 10-10 M by adsorptive stripping square wave voltammetry. The developed method was applied to an injectable dosage form for the determination of leuprolide acetate with satisfactory accuracy and precision.