Investigation of Antioxidant and anti-melanogenic Activities from Secondary Metabolites of Endophytic Fungi Isolated from Centella asiatica and Syzygium polyanthum

This study explores the potential of natural-based and environmentally friendly ingredients for functional cosmetics by investigating the antioxidant and anti-melanogenic activities of endophytic fungi associated with Centella asiatica (CA) and Syzygium polyanthum (SP).Three endophytic fungi were isolated from leaves and stems of CA and SP collected from Bogor and Serpong, Indonesia using Rose Bengal agar medium.The fungal isolates were cultivated through submerged fermentation in malt extract broth medium.After separating the mycelial biomass with Whatman filter paper, the supernatant was extracted using ethyl acetate.The ethyl acetate fraction of the mycelial biomass extract from these three fungal isolates was screened for antioxidant and anti-tyrosinase activities.In the preliminary screening using the DPPH method by thinlayer chromatography (TLC) with a mobile phase of ethyl acetate-methanol (9:1, v/v) and toluene-ethyl acetate (9:1, v/v), strong yellow spots indicating antioxidant activity were observed from each isolate after being sprayed with DPPH reagent.Screening for anti-tyrosinase activity was conducted using a colorimetric assay with button mushroom tyrosinase, kojic acid as a positive control and 3,4-dihydroxyphenylalanine (L-DOPA) as a substrate.One isolate with higher antioxidant and antityrosinase activities was further identified based on sequence data of the internal transcribed spacer (ITS) rDNA (including ITS1, 5.8S rDNA, and ITS2).The identification of the selected isolate showed 99.45% similarities with Colletotrichum sojae.