Discovery of Cyclic Lipopeptides, Octaminomycins C and D, by Engineering LysR Transcriptional Regulator of <i>Streptomyces</i> sp.
New lipodepsipeptides, octaminomycins C and D (<b>1</b> and <b>2</b>), were discovered in an engineered <i>Streptomyces</i> sp. strain overexpressing the LysR transcriptional regulator family. The structures of <b>1</b> and <b>2</b> were elucidated by comprehensive analysis of their UV, HRMS, and NMR data. The absolute configurations were determined using spectroscopic analysis, the advanced Marfey's method, and genomic analysis. Genomic analysis enabled the identification of the nonribosomal peptide synthetase (NRPS) biosynthetic pathway of the octaminomycins.
Authors
- Dong‐Chan Oh (ORCID: https://orcid.org/0000-0001-6405-5535)
- Joon Soo An (ORCID: https://orcid.org/0000-0002-3282-5790)
- Jae‐Ho Han (ORCID: https://orcid.org/0000-0002-6490-9825)
- Sang Kook Lee (ORCID: https://orcid.org/0000-0002-4306-7024)
- Yeo Joon Yoon (ORCID: https://orcid.org/0000-0002-3637-3103)
- Sang‐Jip Nam (ORCID: https://orcid.org/0000-0002-0944-6565)
- Jung Min Kim (ORCID: https://orcid.org/0000-0002-0983-9414)
- Jun Young Lee (ORCID: https://orcid.org/0000-0003-2670-165X)
Institutions
- Ewha Womans University (KR)
- Seoul National University (KR)
- Ewha Womans University Medical Center (KR)
Publication Details
- Journal
- Journal of Natural Products
- Published
- 2024-12-13
- DOI
- https://doi.org/10.1021/acs.jnatprod.4c01047
- Citations
- 1
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.45
Funders
- National Research Foundation of Korea