Protocol for isolation and transplantation of rat pancreatic islets in the kidney capsule to treat diabetes
Islet transplantation offers promise for those with type 1 and late-stage type 2 diabetes; however, islet isolation from the pancreas is challenging due to the interference of exocrine tissue. We describe steps for constructing a diabetes model and precise islet isolation by a perfusion-based technique, followed by gradient centrifugation and cultivation in RPMI 1640 for recovery. Transplanted rat islets in diabetic nude mice demonstrated functional recovery, confirming the effectiveness of this approach.
Authors
- Bingbing Sun (ORCID: https://orcid.org/0000-0002-5444-5078)
- Weijing Kong
- Kai Wang (ORCID: https://orcid.org/0000-0001-6240-0147)
- Wei Zhang (ORCID: https://orcid.org/0000-0002-3167-0002)
- Xi Wang (ORCID: https://orcid.org/0009-0004-9115-8539)
- Dayan Li
- Yi Hong
Institutions
- Peking University (CN)
- Peking University Third Hospital (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-13
- DOI
- https://doi.org/10.1016/j.xpro.2024.103486
- Citations
- 4
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 2.29
Funders
- National Natural Science Foundation of China
- Peking University
- Southeast University
- Beijing Nova Program
- Fundamental Research Funds for the Central Universities